演題概要

オーラルセッション

第２日　５月１９日（木）　9:40～10:00　A会場（オービットホール）

2A-O1-0940　　PDF

質量分析計を用いる生体内スフィンゴ脂質の安定性評価と臨床領域への応用

(¹東北大メガバンク・ ²東北大院医・ ³AMED-CREST・ ⁴東北大院薬・ ⁵東大院医)
^o三枝大輔^1,2,3・ 王嬌⁴・ 可野邦行^3,4・ Baasanjav, Uranbileg^3,5・ 蔵野信^3,5・ 池田均^3,5・ 矢冨裕^3,5・ 青木淳賢^3,4

Sphingolipids have various physiological functions, and act as lipid mediators. Recently, attention has focused on the distribution of sphingolipids in the tissues and being a biomarker in a clinical region. Recently, MALDI-IMS techniques were established and useful for the distribution analysis of metabolites. However, only MALDI-IMS data could not demonstrate the functional concentration of metabolite. In addition, sphingolipids in tissue slice has to be taken into account degradation of compounds at room temperature during sample preparation. Therefore, we developed a new strategy of combination with LC-MS/MS and IMS technologies for sphingolipids analysis in clinical samples, and analyzed human tissue and plasma samples.