演題概要

ポスター発表

第1日　5月15日（水）　　P会場（多目的ホール）

1P-30　　PDF

LC-HRMSおよびGC-O-HRMSと多変量解析の組み合わせによる水道水生ぐさ臭原因物質の探索

(¹鹿児島大院理工・ ²国保院)
^o新福優太¹・ 高梨啓和¹・ 中島常憲¹・ 秋葉道宏²

Few papers have been reported so far which successfully identified a causative compound of fishy-smell, the second most common off-flavor of tap water in Japan.The objective of this study is therefore to explore a causative compound of fishy-smell and partially estimate its structure. To achieve this, raw water samples and cultured medium with Uroglena americana (U.americana), a well-known causative organism, were analyzed by liquid chromatography–high resolution mass spectrometer (LC-HRMS), gas chromatography-olfactoametry-high resolution mass spectrometer (GC-O-HRMS), differential analysis, and interval Orthogonal Projections to Latent Structures regression analysis (iOPLS). As a result, one candidate was acquired and its molecular formula was estimated to be C₁₃H₂₀O₃ based on its accurate mass and natural isotopic pattern. The candidate was both detected in the raw water and the cultured media. The GC retention time of the candidate was 9.65 min, and seaweed smell was observed by GC-O analysis at around the retention time. It was suggested by LC-MS/MS analysis that the candidate contain hydroxy group, methoxy-substituted cyclohexane, and enol moiety.