The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 1, May 15（Mon.）　　Room P (Foyer, Room 1004-1007)

1P-38　　PDF

Pretreatment Method for Serum Amino Acids and Short-chain Fatty Acids in Comprehensive Metabolomics

(1Toyama Pref. Univ., 2Kanazawa Univ.)
oKaito Yamasaki1, Shigehiro Karashima2, Issey Osaka1

We developed pretreatment methods for free amino acids and short-chain fatty acids for in comprehensive metabolomic analysis. A large amount of 3-nitrophenylhydrazine was used as a derivatization reagent for them. As a result, the reagent was detected as high ion intensity signal in obtained LC/MS chromatogram. Conventionally, solid-phase extraction was applied for the removal of the derivatization reagent. However, it is difficult to sufficiently recover and detect the various derivatized free amino acids and short-chain fatty acids without separating completely from the derivatization reagent. Therefore, we investigate the method for removal of excess unreacted derivatization reagent in comprehensive metabolomics.