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Late Breaking Posters
Day 1, June 22（Sun.）
Room P (Maesato East, Foyer, Ocean Wing)
- 1P-LB-01
Measurement of Metals in Human Liver Using LA-ICP-MS and an Organic Matrix-Based Standard
(1Agilent, 2Kyoto Univ., 3Tokushima Univ.)
oTetsuo Kubota1, Yuki Sugiura2, Koichi Tsuneyama3
Bioimaging is essential for visualizing tissues, cells, and compounds, while metallomics studies highlight the roles of metals in biological systems by visualizing distribution of metals in tissues. Laser Ablation-Inductively Coupled Plasma-Mass Spectroscopy (LA-ICP-MS) can provide localization information of elements on solid samples. Therefore, bioimaging techniques using LA-ICP-MS have been developed to investigate the distribution of metals on thin sections.
Quantifying metals with LA-ICP-MS involves three methods: (1) using inorganic matrix-based standards, (2) using element-spiked tissue samples, and (3) normalizing to bulk metal components as 100%. For the first method, commercially available solid reference materials are unsuitable due to discrepancies in ablation efficiency and matrix effects of the sample aerosol when compared to carbon-based biological tissues. The second method requires advanced skills and is time-consuming. And the challenge of the third method is scanning all elements within a single ablated spot. An organic matrix-based standard has emerged as a promising solution. The aim of the study is to directly quantify metals such as Mg, P, Ca, Fe, Cu, and Mo in human liver specimens using LA-ICP-MS, a highly sensitive technique. The elemental concentrations were determined from external calibration curves generated using an organic matrix-based standard (Solid scaleTM, FUJIFILM Wako Chemicals).