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Late Breaking Posters
Day 1, June 22（Sun.）
Room P (Maesato East, Foyer, Ocean Wing)
- 1P-LB-04
Proteomic analysis of corneal layers after trigeminal denervation: insights into inflammation and intracellular clearance in the epithelium, stroma, and endothelium
(1NTUH, 2NTU)
oPei-Shan Wu1, I-Lan Tsai1, Miao-Hsia Lin2, Hsin-Yu Liu1
The cornea, highly innervated by sensory nerves from the trigeminal nerve, interacts dynamically with various cell populations. Impaired innervation can cause disorders like neuropathic keratopathy, leading to significant vision loss. Most research has focused on the corneal epithelium, while the involvement of other corneal layers, vital in later stages of the disease, has often been overlooked. To investigate the impact of denervation on different corneal cell populations, a total of 2,000 cells were sorted individually from the corneal epithelium, stroma, and endothelium of normal or denervated mice, and then collected for DIA proteomics analysis. Statistics and bioinformatics analysis revealed that proteins up-regulated in the denervated cornea were involved in inflammatory response across all three layers, along with distinct alterations in nucleotide and energy metabolism in different layers. In contrast, the pathways enriched in down-regulated proteins were associated with biological oxidation and vesicle transport in both epithelium and stroma, and associated with apoptosis execution phase and ECM organization in endothelium. These results suggest that following corneal denervation, different phases of inflammation occurred in the three corneal layers, along with a diminished ability for intracellular clearance of apoptotic cell. These findings provide new insights into neurotrophic keratopathy's pathophysiology and advance therapeutic strategies.