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Late Breaking Posters
Day 1, June 22（Sun.）
Room P (Maesato East, Foyer, Ocean Wing)
- 1P-LB-06
Analysis of Sulfated N-glycans as a Potential Biomarker for the Early Detection of Breast Cancer
(1Hokkaido Univ., 2De La Salle Univ., 3Addis Ababa Univ)
oDereje Feleke1, Bryan Montalban2, Solomon Gizaw3, Hiroshi Hinou1
Background: Breast cancer (BC) remains a leading cause of mortality among women, and early detection significantly improves patient outcomes. Glycosylation changes, particularly sulfation, are implicated in cancer progression but are challenging to analyze due to low abundance. This study aimed to profile sulfated N-glycans in Ethiopian BC patients to identify novel early-stage biomarkers.
Methods: A glycoblotting-based sulphoglycomics workflow was employed, integrating glycoblotting enrichment, weak anion exchange (WAX) separation, and MALDI-TOF MS. Serum samples from 76 BC patients and 20 age-matched healthy controls were analyzed. Statistical tools assessed differences in sulfated N-glycan profiles and their diagnostic potential.
Results: We identified seven mono-sulfated N-glycans with significantly elevated levels in BC patients, showing high diagnostic accuracy (AUC ≥ 0.8). Terminal Lewis-type glycan epitopes were predominantly observed in sulfated glycans. Increased total fucosylation and sialylation levels on sulfated glycans were also significant markers for early-stage BC. Sialic acids were preserved during analysis, allowing comprehensive evaluation.
Conclusion: This is the first quantitative study of sulfated N-glycans in BC patients, identifying novel glyco-biomarkers with strong discriminatory potential. These findings emphasize the clinical relevance of sulfated glycans in BC diagnostics, warranting further validation in larger cohorts.