| Timetable |
| Download Conference Program |
| Download All Abstracts |
| Zoom Access |
| Corporate Program |
Late Breaking Posters
Day 1, June 22（Sun.）
Room P (Maesato East, Foyer, Ocean Wing)
- 1P-LB-11
Development of a sheathless CE-MS method for limited sample volume
(Keio Univ.)
oAkiyoshi Hirayama, Yushi Kamei
One of the ultimate goals for researchers involved in metabolomics is to detect metabolites contained in a single cell. Unlike conventional methods that collectively measure and analyze multiple cells, it is expected that more detailed analysis that reflects the individuality of each cell will be possible.
Capillary electrophoresis-mass spectrometry (CE-MS) is a powerful tool for comprehensive analysis of charged compounds. At present, sheath flow interface is the most commonly used technique in CE-MS. However, this interface leads to decrease in sensitivity due to dilution of analytes with sheath liquid. To improve the sensitivity with CE-MS, we have developed a novel sheathless CE-MS interface.
By using this system, we successfully detected 53 types of cationic metabolites. On the other hand, it was difficult to measure anionic metabolites with the original configuration. When the cause of this was investigated, it was found that discharge was occurring in the ion source. Therefore, we developed a new analytical method using sheath gas. As a result of optimizing the gas composition and flow rate, it was possible to successfully detect over 60 types of anionic metabolites.
This interface could be a powerful new tool for highly sensitive CE-MS-based metabolome analysis.