Abstract

Poster Presentations

Day 1, June 22（Sun.）　

Room P (Maesato East, Foyer, Ocean Wing)

1P-PM-06
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Metabolome Analysis of Juvenile Corals using a Comprehensive Two-Dimensional Gas Chromatography High-Resolution Time-of-Flight Mass Spectrometry for Calcification-Related Compounds

(¹JEOL, ²Kitasato Univ., ³AIST, ⁴Okayama Univ., ⁵Univ. Ryukyus)
^oAzusa Kubota¹, Ayumi Kubo¹, Masaaki Ubukata¹, Nanami Mizusawa², Mariko Iijima³, Yoshikazu Ohno⁴, Jun Yasumoto⁵, Ko Yasumoto²

Corals build skeletons from calcium carbonate, but the mechanism remains unclear. By comparing metabolites between planula larvae and primary polyps, we aim to clarify this process. It is recognized that biological metabolites consist of many compounds. Therefore, using a comprehensive two-dimensional gas chromatography high-resolution time-of-flight mass spectrometry (GCxGC-HRTOFMS), we analyzed metabolites extracted from juvenile corals. Electron ionization (EI) and field ionization (FI) methods were employed for ionization in the GCxGC-HRTOFMS measurements. Data processing and qualitative analysis were performed using msFineAnalysis AI. Compound identification was achieved through a NIST database (DB) search and accurate mass analysis. A machine learning-generated EI mass spectra library was utilized to estimate structures for compounds not listed in the NIST DB. The GCxGC measurements facilitated peak separation, and metabolites were identified, including amino acids and sugars.
In planula larvae, characteristic compounds such as glucose and adenosine were detected, while in primary polyps, 1,3-propanediamine, tyramine, and decanoic acid were identified. Unknown compounds were also found, with one identified as "N,N-bis(trimethylsilyl)pentan-1-amine". The detection of amine compounds in primary polyps suggests their involvement in skeletal formation. This study demonstrates that a machine learning-generated EI mass spectra library can infer structures of unregistered compounds, aiding in understanding coral skeletal formation.