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Poster Presentations
Day 1, June 22（Sun.）
Room P (Maesato East, Foyer, Ocean Wing)
- 1P-PM-08
BIOSP-Based Multi-Scale Proteomics Decodes the Adipose-Brain Axis in Obesity
(BMS CITYU)
oRui Qian, Fenglian Yang, Liang Zhang
This study developed an innovative technology integrating proximity-dependent biotinylation (BioID), mass spectrometry (MS), and proximity ligation assay (PLA) -termed BIOSP to achieve high-throughput proteomic analysis coupled with in situ localization of target proteins. By fusing biotin ligase with target proteins through BioID, proximity-dependent biotinylation enables streptavidin-based purification and high-resolution MS identification of protein interaction networks in mouse adipose tissue. This MS-based approach successfully identified obesity-related regulatory factors and we further developed BIOPLA technology to enable in situ detection of obesity-related regulatory factors. We also revealed region-specific leptin distribution patterns in mouse brain tissues, with protein abundance significantly correlating with obesity phenotypes using BIOSP to investigate the adipose-brain axis in obese mouse models. This integrated platform provides a comprehensive solution spanning "panoramic screening-precise quantification-spatial mapping" for proteomic research, while establishing novel methodologies for studying inter-organ communication in metabolic diseases. The findings elucidate dynamic leptin transport mechanisms between adipose and brain, providing theoretical foundations for optimizing therapeutic targets against obesity. The BIOSP system not only advances proteomic analysis capabilities but also demonstrates exceptional potential for multi-scale investigation of protein networks in complex biological systems.