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Poster Presentations
Day 1, June 22（Sun.）
Room P (Maesato East, Foyer, Ocean Wing)
- 1P-PM-18
Multi-omics analysis of CHO cells for improvement of monoclonal antibody production process
(AGC Inc.)
oShigenori Takeda, Yumi Yamanaka, Kana Tanabe, Yasuhiro Kawano, Nobuyoshi Nagao
Chinese hamster ovary (CHO) cells are widely used to produce biopharmaceutical proteins. In our previous report, proteome and transcriptome analysis found that cystine and tyrosine (Tyr) feeding improved growth, viability, and monoclonal antibody (mAb) productivity of CHO cells owing to suppression of oxidative stress and apoptosis. Understanding molecular mechanisms of CHO cells cultured with the addition of cystine and Tyr is expected to be applicable to media optimization, thereby contributing to improve the mAb production process. However, the detailed molecular mechanisms remain unclear. Here, we have developed simple multi-omics methodology and clarified quality factor for mAb productivity. First, the constructed methodology with data independent acquisition (DIA) proteome analysis could detect 8,000 proteins which seems enough for analysis of intracellular proteins. Second, we conducted comparative experiments with (CT feed) and without (Control) cystine and Tyr feedings using non-targeted metabolome, non-targeted lipidome, and DIA proteome analyses. Proteome and metabolome analyses indicated that tricarboxylic (TCA) cycle and glutathione metabolomics were activated in CT feed condition. Finally, to activate TCA cycle more, the addition of metabolites suggested by extracellular metabolome analysis to be insufficient resulted in an increase in productivity.