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Poster Presentations
Day 1, June 22（Sun.）
Room P (Maesato East, Foyer, Ocean Wing)
- 1P-PM-26
Identification of Pannexin-3 as a Missing Protein in the Human Proteome Using Liquid Chromatography-Tandem Mass Spectrometry
(1KBSI_DORC, 2KMU_DBC, 3KMU_ARI, 4KBSI_BRC, 5KRIBB, 6UST)
oHae Min Ju1, Kyung Hee Kim2,3, Soojin Park4, Jin Young Kim1,5, Heeyoun Hwang1,6
The Human Proteome Project (HPP) has classified Pannexin-3 (PANX3) as a "missing" protein due to insufficient evidence of its existence. PANX3, a member of the pannexin family, is known to form functional cell surface channels and play roles in various physiological processes. This study finds the PANX3 (NX_Q96QZ0, Protein Evidence Level 2) located on chromosome 11 using liquid chromatography-tandem mass spectrometry. We discovered PANX3 from cancer patients, and subsequently validated through comparison with corresponding synthetic peptides. This approach not only confirmed the presence of PANX3 but also demonstrated the effectiveness of LC-MS/MS in identifying previously undetected proteins. We provide an evidence for PANX3's existence and establishes a foundation for further investigations into its biological functions and potential clinical significance. Our findings contribute to the ongoing efforts of the HPP to comprehensively characterize the human proteome and highlight the importance of advanced analytical techniques in protein identification.
This study was supported by the Korea Institute of Marine Science & Technology Promotion (Grant no. RS-2024-00405273)