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Poster Presentations
Day 1, June 22（Sun.）
Room P (Maesato East, Foyer, Ocean Wing)
- 1P-PM-33
Spatial Lipidomics Mapping in 3D Cell Models via DESI Mass Spectrometry Imaging
(Chang Gung Univ.)
oChen Yu Chang, Cheng Hung Yang
To better mimic in vivo biological processes, cell culture methods have evolved from traditional monolayers to three-dimensional (3D) models. These models provide deeper insights into the heterogeneity, structure, and functionality of cancer cells, bridging the gap between in vitro and in vivo systems. However, conventional mass spectrometry preprocessing often overlooks spatial metabolic differences. Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI-MSI) allows direct chemical analysis from tissue surfaces without preprocessing, enabling 2D mass spectral mapping of sliced spheroids.
In this study, HepG2 cells were cultured into spheroids, cryosectioned, and analyzed using DESI-MSI to investigate lipid differences between inner and outer cell layers. The results indicate that outer cells contain more triglycerides, diglycerides, monoglycerides, and cholesterol esters, while inner cells exhibit higher levels of phosphatidylcholine and phosphatidylethanolamine. This approach enhances our understanding of cancer cell metabolism in 3D environments and allows further exploration of how gene expression or drug treatments affect lipid distribution within spheroids.
By establishing a lipidomic database for spheroids, this study provides a foundation for future investigations into the metabolic dynamics of cancer cells, helping to bridge the gap between in vitro experiments and clinical drug development.