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Poster Presentations
Day 1, June 22（Sun.）
Room P (Maesato East, Foyer, Ocean Wing)
- 1P-PM-42
Spatial multi-omics study delineates possible link between copper dyshomeostasis and macrophage driven inflammation in Dilated Cardiomyopathy
(1Doshisha Univ., 2SHIMADZU, 3Kyoto Univ., 4NCNP, 5NCVC, 6Hokusetsu General Hospital)
oMaiko Okamura1, Koji Okuda2, Shinichi Yamaguchi2, Takushi Yamamoto2, Kenji Minatoya3, Shuji Yamashita1, Ichizo Nishino4, Hatsue Ishibashi-Ueda5,6, Masaya Ikegawa1
Dilated cardiomyopathy (DCM) is a group of progressive, poor prognosis diseases that primarily consist of ventricular enlargement and myocardial contractile dysfunction. The immune response plays a pivotal role in cardiovascular disease development and tissue macrophages play an essential role in the initial inflammatory response. J2N-k hamsters (J2N-k), which have a defective δ-sarcoglycan (δ-SG) encoding gene, have been utilized as a suitable DCM animal model. By our original observations, detailed phenotypes of J2N-k in myocardium and skeletal muscles have been histopathologically clarified. Of note, inflammation, fibrosis, calcification and appearance of multi-nucleated giant cells in myocardium were noticeable in the early to mid-stage of J2N-k. In this study, we adopt mass spectrometry imaging based spatial multi-omics study to delineate immunometabolism on DCM animal model. For the same slides, <I>in situ<I> visualization and estimation of the concentration of <I>in situ<I> phosphorus (P), calcium (Ca), magnesium (Mg), iron (Fe), zinc (Zn), and copper (Cu) was successfully performed with in situ metabolites. In understanding detailed immunological phenotypes of the current model to merge metabolomic and elemental mass imaging, photo-cleavable mass-tagged antibodies against CD4, CD8, and CD3e for T cell, CD20 for B cell, and CD68 for macrophage marking were used for MALDI HiPLEX-IHC.