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Poster Presentations
Day 1, June 22（Sun.）
Room P (Maesato East, Foyer, Ocean Wing)
- 1P-PM-44
Reliability on MS image of citric acid distribution in a strawberry fruit specimen created using positive/negative ion mode associated with potassium distribution
(1Hokkaido Univ., 2Shimadzu)
oTakumi Fujiki1, Kaoru Nakagawa2, Koji Okuda2, A. Tanabe2, M. Kiyama2, Manami Kobayashi2, Takashi Suzuki1
MALDI MS imaging (MSI) is a technique that can visualize the distribution of compounds in a tissue specimen. It is known that the MS image created using positive ion mode of MSI is sometimes different from that of negative ion mode. So, we hypothesized that this difference might be due to the uneven presence of potassium, especially in the case of positive ion mode, where molecules were ionized by attaching a cation. We used a multimodal imaging system to analyze the distribution of citric acid and potassium ion in a strawberry fruit specimen. The distribution of citric acid determined using positive ion mode did not coincide with that of potassium ion. Then, the adjacent fruit tissue was divided into twelve sections and the citric acid content was quantified using LC. The coefficient of determination between the citric acid content quantified using LC and the mean intensity of citric acid in each section of the MS image was calculated. As a result, the coefficient of determination was rather higher in the MS image from positive ion mode than negative. Thus, ionization of a compound seems not be influenced by uneven distribution of cation regardless of the positive/negative mode.