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Oral Sessions
Day 2, June 23（Mon.） 11:55-12:10
Room A (Maesato West)
- 2A-O1-1155
Analysis of Highly Multiply Charged Intact Polymers in Cyclic Ion Mobility-Mass Spectrometry with Collision Induced Charge Stripping
(NITech)
oIsa Guducu, Iiguni Yoshinori, Shinya Kitagawa
Polymers with large molecular weight are difficult to interpret in ESI-MS due to the generation of various highly multiply charged analytes and their overlap. To overcome this problem, we have developed a method that combines MS/MS, ion mobility (IM), and collision-induced charge stripping (CICS) for the analysis of large intact polymers. In this method, the key is the separation in the 2D map for arrival time and m/z. Thus, increasing the separation efficiency in IM would increase the potential to analyze large intact polymers. Recently, a high resolution IM called “cyclic IM" has been developed and is commercially available. In this study, the analysis of highly multiply charged intact polymers using cyclic IM-MS with CICS is fundamentally investigated. Here, the analysis of polyethylene oxides in IM-MS/MS with CICS was investigated. As reported in our previous work, the CICS method was effective in separating PEOs of selected m/z in the 2D plane due to the difference in arrival time and m/z after the charge reduction. Although the signal intensity decreased, the separation of highly charged polyethylene oxides were improved with the increase in the number of IM cycle.