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Oral Sessions
Day 2, June 23（Mon.） 15:10-15:25
Room A (Maesato West)
- 2A-O2-1510
Quantitative metabolomics for human plasma using stable isotope-labeled internal standard mixture (SILIS)
(1MIB, Kyushu Univ., 2Kyushu Univ., 3AIST, 4Taiyo Nippon Sanso, 5Keio Univ., 6SAIL Technologies)
oMasatomo Takahashi1,2, Yuki Soma3, Akari Ikeda4, Akiyoshi Hirayama5, Kanako Tokito1, Michiyo Hishikawa6, Satsuki Ikeda5, Yuri Imado2, Tsutomu Terauchi6, Takayoshi Matsuda6, Yoshihiro Izumi1,2, Takeshi Bamba1,2
With the recent development of artificial intelligence, the use of large-scale data from various omics such as transcriptomics, proteomics, metabolomics has attracted attention in the field of life science research. However, in order to perform integrated analysis using metabolome data, a method is needed that allows the integration of metabolome data acquired by different instruments in different facilities. A stable isotope dilution method (i.e. use of authentic stable isotope-labeled standards for each metabolite of interest) has been proposed as one of the methods to address this issue. However, commercially available stable isotope standards are usually too expensive and labor-intensive to prepare a stable isotope-labeled standard mixture for all metabolites of interest. We have developed the biological preparation of stable isotope-labeled internal standard mixture (SILIS), which can stably and inexpensively produce a wide variety of metabolites, by culturing E. coli with 13C6-glucose as a sole carbon source. SILIS is expected to enable quantitative metabolomics and reduce variability in inter-laboratory comparisons. In this study, we report the results of quantitative metabolomics of human plasma (SRM1950) using SILIS in multiple institutions.