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Oral Sessions
Day 2, June 23（Mon.） 16:25-16:40
Room A (Maesato West)
- 2A-O3-1625
Hybrid Imaging Analysis reveals molecular localization reflecting brain function
(1Dokkyo Medical Univ., 2QST, 3Tokyo Univ. Pharm. Life Sci., 4Univ. Tokyo)
oTadayuki Ogawa1, Shino Takeda2, Tomonari Umemura3, Takafumi Hirata4
The slice sequences of the spinal cord were analyzed by the hybrid imaging composed of the conventional histological analyses and SEM with MALDI -MSI, PIXE imaging, HE-SR-XRF imaging, and LA-ICP-MSI. From a large number of spectral data sets, the specific molecular distribution was verified by mathematical and image analysis, including Principal Component Analysis. In addition, we have developed an improved sectioning method for MS-imaging analysis of biological samples that are difficult to section. MALDI-IMS revealed the specific localization of phospholipids, monoamines and nucleotides in the functional regions such as white matter, gray matter, anterior horn, and dorsal horn. PIXE clearly visualized the distribution of the atoms such as phosphorus and potassium. In addition, HE-SR-XRF detected the localization of iron and zinc in the dorsal horn. LA-ICP-MS imaging quickly revealed a wide range of metal distributions in the wide field of the spinal cord. The integrated image data showed the specific co-localization and distribution of molecules and elements. PCA analysis showed that the spectral data represented well-differentiated functional and anatomical regions within the spinal cord, such as dorsal horn, ventral horn, gracile fasciculus, cuneate fasciculus, lateral spinothalamic tract. This demonstrates that the molecular localization is a reflection of brain function.