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Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-AM-09
De Novo Structural Elucidation of Cnidarian Metabolites by Non-Targeted LC-ESI-MS/MS
(1OIST, 2Kyushu Univ., 3Osaka Univ., 4Miruion Inc.)
oYayoi Hongo1, Hiroshi Watanabe1, Kenji Hamase2, Chiharu Ishii2, Shuichi Shimma3,4, Hiromi Saito4
Cnidaria holds a pivotal position in early animal evolution, serving as the closest sister group to bilaterians. While cnidarian genomes have been extensively studied in evolutionary neurobiology, their endogenous metabolite repertoire remains largely unexplored. Here, we applied non-targeted LC-ESI-MS/MS to characterize cnidarian metabolites in Nematostella vectensis and Hydra vulgaris. Lyophilized samples were extracted using a modified Bligh-Dyer method, and analyzed via LC-ESI-MS/MS with an Orbitrap QE-HF system. A hybrid ion-exchange/C18 column enabled detection of diverse small metabolites including urea. Structural elucidation was achieved by comparing MS/MS spectra with known standards. Unique amino acid modifications contributed to the distinct diversity of cnidarian metabolites, differentiating them from conventional model organisms. Additionally, chiral amino acids were analyzed. Some of these metabolites, functioning as signaling molecules in higher animals, will be further investigated via imaging mass spectrometry (IMS) and bioassays to assess their biological activities.