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Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-AM-14
Matrix-Enhanced SALDI Imaging of Consecutive Tape Strips for Visualizing Skin Penetration of Cosmetic Ingredients
(KOSÉ Corporation)
oYi Lyu, Shunichi Suga, Masatoshi Sekiya, Daiki Yamakoshi, Ken Tanaka, Tsuyoshi Hata
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a powerful technique for detecting a wide range of molecules while preserving spatial information, offering significant potential for skin penetration analysis in cosmetic research. Tape stripping is a widely used approach to sample the epidermis using adhesive tapes. However, applying MALDI-MSI to adhesive tapes is challenging due to their non-conductivity, thickness, and outgassing. To address these issues, we developed an optimized method using matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS), a hybrid method of MALDI and SALDI that uses both organic matrices and metallic nanoparticles. This method enables stable MS imaging over several hours while maintaining sufficient ion intensity. MSI analysis of consecutive tape strips demonstrated a clear decrease in target ingredient levels from the upper to lower stratum corneum (SC) layers, consistent with LC/MS/MS quantitative data. Furthermore, differences in skin penetration profiles were observed between different cosmetic formulations. These findings highlight the potential of MSI with tape stripping as an effective tool for skin penetration studies targeting the SC, as well as for efficacy analysis and safety assessment in cosmetic development.