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Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-AM-17
Streamlining LC-MS Sample Preparation through 3D Printing Technology
(Sogang Univ.)
oHwa-Yong Jang, Han Bin Oh
We developed an advanced robotic system to improve the efficiency and precision of sample preparation for liquid chromatography-mass spectrometry (LC-MS) analysis. Using open-source 3D printing technology, we reduced development costs and time while ensuring scalability and adaptability. Manual sample preparation often suffers from inefficiencies, poor reproducibility, and standardization challenges. To overcome these, we designed an automated platform with three-axis motion control, G-code automation, and a 3D printer control board, powered by Klipper firmware and a custom web interface for enhanced accuracy and usability. The system integrates three modules: the Automated Liquid Handling System (ALHS), the Automated Reagent Storage System (ARSS), and the Automated Consumable and Equipment Storage System (ACESS), minimizing human intervention and improving repeatability. We validated the system by automating the TEMPO-FRIPS peptide sequencing protocol, achieving faster preparation and better consistency in peptide quantification compared to manual methods. All designs, software, and documentation are openly shared to encourage adoption and customization. This work, supported by the National Research Foundation of Korea (2021R1A2C2007397), the Ministry of Education, and KEITI (RS-2023-00219144), demonstrates the potential of cost-effective, modular automation to enhance laboratory workflows.