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Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-AM-21
Phosphoproteomic Analysis Reveals the Role of HAESA in IDA-Mediated Lateral Root Development
(1NCKU, 2IPMB, Sinica)
oLi-Hsuan Yu1, I-Fan Wang1, Kuan-Hao Huang1, Chuan-Chih Hsu2, Ying-Lan Chen1
Lateral root development is essential for water and nutrient uptake in plants. In Arabidopsis thaliana, this process is tightly regulated by receptor-like kinases such as HAESA (HAE) and HAESA-LIKE2 (HSL2), which perceive the peptide ligand INFLORESCENCE DEFICIENT IN ABSCISSION (IDA). Using a split-root system, we recently demonstrated that local application of IDA promotes both local and systemic lateral root development, highlighting its role in long-distance signaling. Interestingly, lateral root emergence in the hae mutant is insensitive to IDA treatment. However, the hae mutant exhibits a lateral root number comparable to IDA-treated wild-type plants, suggesting that HAE functions not only as an IDA receptor but also as a negative regulator of lateral root emergence at low IDA concentrations. To investigate this potential inhibitory role of HAE, we performed phosphoproteomic analyses comparing wild-type and hae mutant plants under local IDA treatment. Our analysis of phosphoproteomic changes between wild-type and the hae mutant, using both local IDA-treated and remote untreated roots, revealed distinct phosphorylation pathways involved in lateral root development and salt stress responses. By comparing these phosphoproteomic datasets, we aim to elucidate how IDA-HAE signaling regulates lateral root development and uncover the molecular mechanisms governing long-distance root communication.