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Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-AM-40
INSIGHTS INTO DIET AND EXERCISE INDUCED CHANGES IN ZEBRAFISH LIPIDOME VIA LC-MS AND CHEMOMETRIC ANALYSES
(1IQ-UFG, 2UFLA, 3DB-UFPR, 4DQ-UFPR)
oAndrea Chaves1, Almir Batista Junior1, Jussara Roque1, Lanaia Ítala Maciel1, Moises Martins2, Willian Carneiro2, André Viana3, Luis David Murgas2, Ricardo Bernardo4
Understanding molecular-level metabolic changes associated with obesity can contribute to the prevention of various clinical conditions. For such, zebrafish (Danio rerio) has emerged as a robust model for studying lipid profile variations due to its genetic and physiological similarities to humans. Hence, this study aimed to investigate lipid alterations in zebrafish models under different dietary and exercise conditions through liquid chromatography-mass spectrometry (LC-MS) and chemometrics. Lipids were extracted using a liquid-liquid microextraction protocol based on the Bligh & Dyer method. Chromatographic separation was performed in reversed phase (C18 column at 60 °C), with 5 µL of sample injected into a mobile phase at a flow rate of 0.300 mL·min⁻¹. The mass spectrometry analyses were acquired in both positive and negative ionization modes using an electrospray ion source. LC-MS results provided distinct metabolic profiles for each experimental condition, enabling the identification of significant distinctions among zebrafish groups and revealing variations driven by diet and exercise. The results exploit that specific lipid classes, such as fatty acids and ceramides, were implicated in obesity-associated metabolic pathways. These lipid classes can be associated with diabetes and cardiovascular diseases and may serve as predictors for these conditions.