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Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-AM-48
Comparison Of Fast Scanning Data Dependent And Data Independent Acquisition Methods For A Multi-OMIC Cancer Study Using High-Speed Chromatography
(Waters Corporation)
oDavina Stewart
Metabolomics and lipidomics allows changes within biological systems resulting from disease, treatment, lifestyle, etc to be investigated. Typically, OMICS analyses are performed using a combination of liquid chromatography (LC) and mass spectrometry (MS). Despite developments in analytical technologies the detection and identification of compounds and subsequent biological interpretation remains a significant challenge. Here we evaluate data dependent (DDA) and data independent acquisition (DIA) modes to demonstrate their utilization for multi-OMIC studies performed using fast chromatographic gradients, and thereby addressing challenges such as spectral complexity, datafile size and quantitation. The benefits of these approach are shown using plasma samples from a colon/rectum cancer pilot study, using the Xevo MRT mass spectrometer with vendor neutral third-party informatic solutions for data processing.