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Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-PM-02
Small-molecule Fingerprinting Discriminated between Geographic Origins of White Shrimp
(1NTU, 2NTOU)
oWen-Ling Chen1, Hsiao-Chi Hu1, Fan-Hua Nan2, Chien-Wei Tu2
This study applied untargeted small-molecule fingerprinting to differentiating between white shrimp (Litopeaneus vannamei)'s geographic origins, which is a major determinant of price but challenging to identify.
The tissue (2 g) of fresh frozen white shrimp was processed with solvent extraction and cartridge cleanup. The small-molecular ions (m/z 70-1100) were acquired using liquid chromatography quadrupole-time-of-flight mass spectrometry in data-independent acquisition mode. Orthogonal partial least squares-discriminant analysis (OPLS-DA) models were built to compare the distribution of small molecules between origins. Markers of origin were identified as fulfilling the requirements of Schymanski Level 2.
OPLS-DA modelling results demonstrated that small-molecule fingerprints in white shrimp differed significantly between the geographic origins (R2Y > 0.6 and Q2 > 0.3; permutation Q2 slope > 0). Lipid decomposition products, including PC(16:0), were more abundant in the imported shrimp (n = 32), compared to shrimp produced in Taiwan (n = 71) (p < 0.05), likely due to prolonged storage duration. Markers of western Taiwan (n = 36), including eicosapentaenoic acid and peptides, that were more abundant than in shrimp from eastern Taiwan (n = 35) (p < 0.05), were associated with low water salinity and earthen pond aquaculture. The markers may be applied to future traceability inspection practices.