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Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-PM-17
PSD Fragmentation Characteristics of Linear and Cyclic O-Linked Glycopeptides and Their Peptide Backbones in MALDI-TOF/TOF MS
(Hokkaido univ.)
oKohki Fukushi, Shogo Urakami, Hiroshi Hinou
O-linked glycans are post-translational modifications where glycans attach to serine or threonine residues in proteins, playing crucial roles in various biological processes. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a technique used to analyze biomolecules, including these glycans. In this study, we analyzed linear and cyclic antifreeze glycoproteins (AFGPs) and their peptide backbones using MALDI-TOF/TOF MS. We observed specific neutral losses: carbon monoxide (28 Da) from ring opening, dehydration (18 Da) at the C-terminus, and losses of water (18 Da) and acetaldehyde (44 Da) from threonine side chains. The prevalence of these losses varied based on factors like the type of adduct ion, glycosylation status of threonine, and substitution with serine. These findings suggest a new fragmentation mechanism for cyclic peptides, offering insights that could aid in discovering biologically active cyclic (glyco)peptides and predicting their functions.