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Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-PM-28
Role of intracellular calcium increase in biological response to cold stress in human hepatoma HepG2 cells
(Chiba Univ.)
oAyano Fukuda, Yoshikazu Yamagishi, Sayaka Nagasawa, Yasumitsu Ogra
Hypothermia is a phenomenon that human body temperature falls below 35ºC. While a deep body temperature below 30℃ causes excessive cold stress, it is used as a protective treatment for brain damage. However, the biological response to cold stress has not been fully understood yet. In this study, we aimed to clarify the biological response to cold stress in HepG2 cells by metabolome analysis with LC-ESI-MS. In the result, taurine, cystine and GSH concentration in HepG2 cells at 30ºC was significantly increased compared with that at 37ºC for 6, 24 and 72 hr incubation. Calcium (Ca) uptake tended to increase at 30ºC, and the taurine, cystine and GSH concentrations were significantly decreased when HepG2 cells were incubated with the Ca-free medium compared with the control medium. In addition, the GSH concentration at 30°C in the medium with MPO inhibitor significantly decreased compared with the control at 30°C. Thus, the biological response to cold stress is assumed to be triggered by an increase in the Ca uptake, followed by the increase in intracellular taurine concentration, and the activation of antioxidant mechanisms such as cystine uptake and GSH production via the Nrf2 activation by taurine-chloramine.