| Timetable |
| Download Conference Program |
| Download All Abstracts |
| Zoom Access |
| Corporate Program |
Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-PM-36
Uniting amplified immuno-mass spectrometry imaging and fluorescent microscopy with lipid imaging by MALDI-MS in murine brain
(1UTS, 2UOW)
oMika Westerhausen1,2, Jayden Mckinnon2, Tassiani Sarretto2, Shane Ellis2, David Bishop1
It is ideal to perform multiomic and multimodal imaging on a single section as it removes the ambiguity of consecutive sections from the imaging pipeline, allowing for straightforward processing and in-depth analysis. Through a combination of atmospheric pressure matrix assisted laser desorption ionisation (AP-MALDI), immunofluorescent microscopy and laser ablation-inductively couple plasma-mass spectrometry (LA-ICP-MS), the biomarker NeuN, a commonly targeted neuronal nuclei protein for histopathological analysis of mature neuronal cells, was investigated within a single murine brain section. The multiplexing of such multi-modal/-omic techniques permitted quantitative, high spatial resolution and unbiased imaging of NeuN and related metabolites spanning multiple metabolic classes. This was realised in a fluorescence segmented LA-ICP-MS quantified hyper stack, which was corelated against the native lipids imaged via AP-MALDI. Image fusion super-resolution of immunofluorescent microscopy and LA-ICP-MS was achieved using bimodal staining of both fluorophore- and lanthanide-conjugated secondary antibodies that exponentially amplified the detected signals. This innovative multiplexed analysis pipeline could obtain unambiguous in-situ spatial localisation of multiple metabolic classes of analytes through a combination of multi-modalities at high granularity from a single section, enabling a deeper understanding of the biochemical landscape, bridging histological and molecular data with unprecedented precision.