| Timetable |
| Download Conference Program |
| Download All Abstracts |
| Zoom Access |
| Corporate Program |
Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-PM-40
Thiol Profiling Based on Live-Cell Derivatization
(CCME, PKU)
oDaiyu Miao, Yu Bai
Endogenous thiols supply vital impetus to physiological process and sustain cellular homeostasis. Assessment of these sulfhydryl-containing metabolites still remains challengeable due to its inherent vulnerability in stability, calling for novel methods which are executed in situ and more sensitive. Though a few works reported that 2-cyanobenzothiazole (CBT) analogues could be utilized for live-cell derivatization of thiols, the nature of CBT-thiol click reaction restricted targets to N-Terminal cysteine residues, limiting the coverage of thiol in their analysis. In this work, a novel live-cell chemical derivatization strategy targeting thiols was developed for accurate quantitation of cellular thiols. Six sulfhydryl targeting probes were designed, synthesized and compared. After derivatization, forty-five thiols were detected and forty among them were assigned to defined structures through ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Benefited from chemical labeling, sensitivity of thiols increased up to 910 folds, with outstanding recovery and reproductivity. Thiols in NE-4C cells varied in concentration over 5 orders of magnitude, with LOD as low as 7.66 pM.