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Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-PM-42
Evaluation of metabolite extraction methods applicable to various yeast species
(1Osaka Univ., 2OTRI, 3Osaka Univ. Shimadzu Lab.)
oHiroaki Ueno1, Ayumu Fuke1, Taisuke Seike1,2, Nobuyuki Okahashi1,2,3, Fumio Matsuda1,2,3
Metabolome analysis is crucial for understanding yeast metabolism and optimizing industrial applications. While metabolomics studies of Saccharomyces cerevisiae were performed by the methanol-chloroform-water (MCW) extraction method , its applicability to other yeast species remains unclear. In addition, chloroform poses safety concerns. This study evaluated a safer alternative, the methyl tert-butyl ether (MTBE)-based method, for three yeast species: S. cerevisiae, Kluyveromyces marxianus, and Schizosaccharomyces pombe. Water-soluble metabolites in the central carbon metabolism were extracted using both methods and analyzed by using LC-MS/MS. The MTBE method identified a comparable number of metabolites. Additionally, cell homogenization efficiency was assessed using propidium iodide staining, revealing that both methods effectively disrupted cell membranes without requiring additional mechanical homogenization. These findings demonstrate that the MTBE-based approach is a viable and safer alternative for metabolite extraction across diverse yeast species.