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Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-PM-44
Exploring of antifungal compounds produced by Streptomyces sp. ES9
(1Grad. Sch. BOST, Kindai Univ., 2TGA Co., Ltd)
oAsahi Kawabe1, Tatsuya Ohike1,2, Ayano Fujisawa1, Tetsuya Matsukawa1, Takashi Ano1, Shin'ichiro Kajiyama1
The control of diseases caused by plant pathogens is an important issue in agriculture. In recent years, the use of microbial materials has been expected to become more widespread due to the environmental impact and low emergence of resistant strain. We isolated the actinomycete Streptomyces sp. ES9 by screening soil microorganisms. This strain was found to exhibit strong inhibitory activity against several phytopathogenic fungi. The objective of this study was to elucidate the mechanism of action of the antifungal activity of ES9 strain.
First, we extracted cultured ES9 with AcOEt, and antimicrobial assay of AcOEt extract showed similar antimicrobial spectra to confrontation culture assay of ES9, suggesting that AcOEt-extracted compound(s) of ES9 should be respoonsible to antifungal activities of ES9. We then investigated whether ES9 produces the same antifungal compounds as its closely related species, Streptomyces kronopolitis. S. kronopolitis and its related species have been shown to produce phoslactomycin F (MW 641.73) and its isomer, leustroducsin A (MW 641.73). However, LCMS analysis showed that neither phoslactomycin F nor leustroducsin A were found in AcOEt extract. The extract was fractionated using a reversed-phase cartridge column. After column treatment, the 80% methanol fraction, which showed antifungal activity, was purified by preparative HPLC. Isolation was now in progress, and detailed results and discussion will be shown in the presentation.