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Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-PM-52
Spatial Lipidomics with High Structural Specificity by Ion Mobility Modulation
(Tsinghua Univ.)
oXiaoxiao Ma, Yao Qian
Lipidomics is the science that studies the structure, function, interaction, and dynamic changes of all lipids in organisms. Spatial lipidomics analysis helps discover new biomarkers and therapeutic targets, making early diagnosis and treatment of diseases possible. In recent years, structural lipidomics technology has attracted much attention because it can explain the intricate structure of complex lipids. However, the traditional approach of structural omics imaging is to use a deterministic sequential MS/MS sampling method, point by point at the whole tissue level. Therefore, it requires a trade-off between the spatial resolution and information content. The key to break this challenge lies in changing the way imaging data is collected: that is, instead of following the deterministic, sequential MS/MS data collection mode, a large amount of DIA-MS/MS data is collected simultaneously for multiple precursors. To this end, we have introduced ion mobility modulation before wide-window MS/MS data collection (MMSD) to achieve rapid separation of ions in time and space, followed by intelligent spectrum deconvolution algorithms for data mining, to develop multi-scale structural lipidomics technologies that can be used for tissue analysis. The MMSD method represents an over 10-fold improvement in MS/MS coverage of lipid imaging compared with traditional imaging methods.