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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-AM-41
Analysis of local distribution and residence time of Nintedanib in fibrotic lung tissue using mass spectrometry imaging
(1Shimadzu Corp., 2Tokushima Univ.)
oTakushi Yamamoto1, Seidai Sato2, Yasuhiko Nishioka2
Nintedanib, a small molecule tyrosine kinase inhibitor, is widely used for the treatment of lung fibrosis. Mass spectrometry imaging (MSI) can visualize the localization of target molecules on tissue sections. In this study, we investigated the local distribution and residence time of Nintedanib in fibrotic lung using MSI and an experimental mouse model of pulmonary fibrosis.
MALDI-TOF MS was used to examine the optimal matrix for Nintedanib detection using standard solutions. Lung fibrosis was induced in mice by subcutaneous administration of bleomycin-A5. Lung tissues were collected 2-12 hours after oral administration of Nintedanib and tissue sections were prepared. After matrix deposition using iMLayer, the lung tissue sections were observed microscopically, and MSI was performed using iMScope QT.
The peak intensities of Nintedanib were gradually enhanced in lung tissues collected 2-6 hours after administration of Nintedanib and attenuated thereafter. The tendency for Nintedanib-derived signals to localize to fibrotic areas was observed in lung tissue collected 4-6 hours after Nintedanib administration.
Nintedanib was shown to localize and distribute in fibrotic lung tissue with a residence time of 12 hours or less. These results also indicate that MSI is a useful technique for evaluating the distribution of antifibrotic drugs in lung fibrosis.