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Plenary Lecture
Day 4, June 25（Wed.） 8:30-9:15
Room A (Maesato West), Room B (Maesato Center), Room C (Top of Yaima)
- 4-PL-0830
Development of CE-MS Metabolomics and Its Application in Cancer
(Keio Univ.)
oTomoyoshi Soga
Metabolomics has become an essential tool in many fields; however, no analytical method has been developed that fully encompasses all metabolites. This is primarily because more than 1,000 metabolites, with similar to different physical and chemical properties, coexist in the cell, complicating their analysis.
Almost all of the metabolites of key pathways are polar and charged species. Focusing on this characteristic, the authors proposed the first capillary electrophoresis mass spectrometry (CE-MS) method for global metabolomic profiling in 2003. CE-MS shows advantageous features such as high resolution, very low sample consumption, and the possibility to easily detect charged compounds. Moreover, CE-MS has demonstrated its better quantification accuracy compared with other analytical platforms.
Recently, we have reported a high-throughput metabolite profiling based on multiple sample injection capillary electrophoresis triple quadrupole tandem mass spectrometry (CE-MSI-MS/MS), which allows sequential 40-sample analysis in a single run.
This lecture presents two examples of the application of CE-MS metabolomics to cancer research. One is to answer the question of when colorectal cancer metabolism occurs and what are the regulatory molecules that reprogram cancer metabolism, and the other is on the development of a high-throughput screening method for colorectal cancer.