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Oral Sessions
Day 4, June 25（Wed.） 11:25-11:40
Room A (Maesato West)
- 4A-O1-1125
Oxygen Attachment Dissociation-Enhanced MALDI Imaging for Spatially Resolved Isomer Identification
(Shimadzu)
oHidenori Takahashi, Satoshi Kasamatsu, Kaoru Nakagawa, Naoto Mishina, Kenta Takigawa, Manami Kobayashi, Kengo Takeshita, Noriyuki Ojima
Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a powerful tool for visualizing the spatial distribution of biomolecules. However, distinguishing structural isomers remains a significant challenge. In this study, we integrate Oxygen Attachment Dissociation (OAD) with MALDI-IMS to achieve isomer-specific molecular imaging.
OAD is a radical-induced ion dissociation technique that utilizes charge-neutral oxygen radicals to selectively fragment molecular ions, providing unique fragmentation patterns for isomer differentiation. By applying OAD to MALDI imaging, we achieved distinct imaging of carbon–carbon double bond (C=C) positional isomers in mouse cerebellum sections, which were previously unresolvable by conventional methods. For example, when mapping the relative intensity of PC 16:0_18:1(n-7)/PC 16:0_18:1(n-9) at m/z 796.525 ([M+K]+), PC 16:0_18:1(n-9) was enriched in the white matter, whereas PC 16:0_18:1(n-7) was localized in the gray matter. These results confirm that OAD enables clear spatial differentiation of C=C positional isomers.
This novel approach enhances the structural specificity of MALDI imaging, providing a powerful tool for isomer-selective molecular imaging in lipidomics and biomolecular research.