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Day 4, June 25（Wed.）　14:25-14:40

Room A (Maesato West)

• 4A-O2-1425
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Development of Medium Vacuum Chemical Ionization (MVCI) for Mass Spectrometry: Micro-Tissue Analysis via Online Coupling of Supercritical Fluid Extraction/Chromatography

(¹Univ. Osaka/MS-Cheminfo., ²Univ. Osaka)
^oToshinobu Hondo¹, Yumi Miyake², Michisato Toyoda²

We developed a medium vacuum chemical ionization (MVCI) tandem mass spectrometry (MS/MS) to analyze small tissue sections directly with minimal sample preparation. MVCI operates at 100 to 300 Pa using oxonium and hydroxide reagent ions generated from water by a corona discharge. It is highly compatible with the supercritical fluid chromatography (SFC). Placing a microscale supercritical fluid extraction (SFE) with online coupled SFC enables direct analysis of single cells or small tissue sections without further sample preparation. We applied this method to study α-tocopherol (α-T) oxidation in Pisum sativum (Pea) leaves during photosynthesis, obtaining light response curves. We observed a significant increase in 8a-hydroperoxy tocopherone and α-tocopheryl quinone after 35 minutes of light exposure. This method enables real-time tracking of α-T oxidation in plant tissue, which plays a role in scavenging reactive oxygen species (ROS).

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