| Timetable |
| Download Conference Program |
| Download All Abstracts |
| Zoom Access |
| Corporate Program |
Oral Sessions
Day 4, June 25（Wed.） 14:40-14:55
Room A (Maesato West)
- 4A-O2-1440
Extending capabilities of Orbitrap-based MS to cold ion spectroscopy for analytical applications
(EPFL)
oOleg Boyarkine, Vyacheslav Kozlovskii, Andrei Zviagin, Vladimir Kopysov
Mass spectrometry (MS) is arguably the most sensitive method in detecting biomolecules, but often fails in distinguishing their isomers and especially in identification of their structures. Spectroscopy reflects structure of molecules on fundamental level, since it can precisely measure optical transitions between their quantum levels, but it is by far less sensitive than MS and requires cooling of molecules to suppress spectral congestion. A recently developed combination of high-resolution mass spectrometry and cold ion photodissociation UV/IR spectroscopy (CIS) benefits from synergy of the two approaches and enables library-based quantification of isomeric molecules and solving primary structure of unknown metabolites. Despite the demonstrated over years high capability of the method, its wide use for analytical applications is hindered by lack of suitable commercial instrumentation.
We will report on design of a compact prototype CIS Addon module, which uses a commercial Orbitrap-based MS Q-Exactive HF (QE) as a platform for UV-MS sensing of cold biomolecular ions. High performance and universality of the new Addon-QE spectrometer will be illustrated by its use for highly accurate quantification of isomeric polypeptides and for solving primary structures of biological metabolites.