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Day 4, June 25(Wed.)
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-AM-08
Optimising the Single Cell Pipeline using a Multi-Reflecting Q-Tof Platform
(1Nihon Waters K.K., 2University of Surrey, 3Waters Corporation)
oThanai Paxton1, Scarlet Ferrinho2, Lee Gethings3, Nyasha Munjoma3, Paul Townsend2, Olivier Cexus2, David Heywood3, Robert Plumb3, Preeti Mourya2, Shazneil Briones2, Clare Mills2, Matt Spick2
The development of cutting-edge mass spectrometers with high levels of sensitivity and resolution has led to single-cell OMICs gaining significant momentum in recent years. Now, previously unanswerable questions can be probed through the distinct measurements of single cells in lieu of a bulk cell average. However, there remains infinite scope for optimisation in single cell OMIC techniques, from sample collection to data analysis. Here we investigate, optimise and present a single cell lipidomic workflow, established with cultured human cell lines using liquid chromatography (LC) coupled to the Xevo™ MRT mass spectrometer (MS).