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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-AM-18
Investigation of Extraction-Ionization Process of t-SPESI by Current Measurement
(1GSS, Univ. Osaka, 2FRC, Univ. Osaka, 3Univ. Yamanashi)
oMengze Sun1, Yoichi Otsuka1,2, Lee Chuin Chen3, Michisato Toyoda1,2
Mass spectrometry imaging (MSI) is an analytical method that is used to measure the distribution of various components in a sample. A higher spatial resolution up to the single-cell scale is still needed to be developed to study the molecular alterations derived from cellular differentiation inside the diseased tissue. We have developed tapping-mode scanning probe electrospray ionization (t-SPESI), a technique for ambient extraction-ionization of a sample using a minute amount of solvent delivered via an oscillating capillary probe. Extraction is performed by the formation of a liquid bridge when the probe is in contact with the sample surface. The molecules dissolved in the liquid bridge are subjected to ESI. To improve the spatial resolution of MSI, the volume of the liquid bridge must be smaller, and an appropriate bias voltage must be applied to the solvent to ensure the ESI process. In this presentation, we introduce the development of a method for simultaneously measuring the current through the solvent in the capillary probe and the size of the liquid bridge in t-SPESI. We will report on the relationship between the size of the liquid bridge and different physical parameters and the mechanisms of the liquid bridge's dynamic changes.