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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-AM-20（3A-O3-1630）
Investigating the distribution of azetidine-2-carboxylic acid (A2C) in plants using HILIC-MS/MS
(1UTS, 2HyMaS, 3UNSW)
oConnor Phillips1,2, Jake Violi3, David Bishop2, Kenneth Rodgers1
Azetidine-2-carboxylic acid (A2C) is a non-protein amino acid and structural analogue of the protein amino acid proline found in a many plant species. A2C induces toxic and teratogenic effects and has demonstrated the ability to misincorporate in place of proline residues in protein synthesis. The potential of A2C to generate immunogenic myelin basic protein through the synthesis of A2C containing ‘neo-epitopes' has been implicated in the pathogenesis of sporadic multiple sclerosis. The aim of this study was to develop and validate a sensitive hydrophilic interaction liquid chromatography (HILIC) tandem mass spectrometry (MS/MS) method to investigate the presence of A2C in a range of plants consumed both by livestock and humans. Lyophilised plant material underwent aqueous extraction and solid phase extraction (SPE) cleanup to examine the presence of free A2C using HILIC-MS/MS. A2C was quantifiable in most of the plant material, with higher amounts found in the Beta vulgaris cultivars, notably the fodder beet and sugar beet. Due to the wide use of these crops as animal fodder, this study highlights the need to better characterize and understand the distribution of A2C in agriculturally significant crops and determine routes and levels of human exposure