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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-AM-21
Identification of clenbuterol metabolites in livestock by LC-Q-Orbitrap-MS
(Chiba Univ.)
oYoshikazu Yamagishi, Yuki Toda, Sayaka Nagasawa, Hirotaro Iwase, Yasumitsu Ogra
Because clenbuterol (CLE) has been found to have anabolic rather than abusive effects as a drug for athletes, unintentional CLE exposure via meat used for fattening livestock has been a problem for athletes. However, there is currently no method to prove unintentional exposure of CLE through the meat. In this study, we use a new analytical method called isotope pattern screening (IPS) for detection of clenbuterol and its metabolites to clarify the metabolic differences between human and livestock by LC-Q-Orbitrap-MS. In the result, the three metabolites (UK-1, UK-2 and UK-4) were detected in the presence of human microsome in vitro. The four metabolites (UK-1–UK-4) were detected in the presence of bovine and chicken microsomes in vitro. The MS spectra showed that these four metabolites (UK-1–UK-4) were oxidative clenbuterol metabolite with m/z values of 293.0818. In addition, the MSMS spectra showed that UK-3 was 1-(4-amino-3,5-dichlorophenyl)-2-(tert-butylamino)ethane-1,2-diol (hereinafter “CLE-L-1"). CLE-L-1 was only detected in the presence of bovine and chicken microsomes in vitro. Thus, CLE-L-1 is a potential biomarker for anti-doping.