| Timetable |
| Download Conference Program |
| Download All Abstracts |
| Zoom Access |
| Corporate Program |
Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-AM-23
Metal-organic framework-based dispersive solid-phase extraction coupled with UPLC-MS/MS for analysis of mono-, di-, and triphosphoadenosine compounds
(1NTU, 2NTNU)
oYu-Meng Wang1, Sung-Fang Chen2
Nucleotides are essential biomolecules that serve as sources of energy in biological systems. Metal-organic frameworks (MOFs), have garnered significant attention due to their high surface area and uniform pore size. The MOFs is an ideal material for adsorp-tion applications, which have demonstrated significant potential of MIL-101-NH2 (Al) for phosphate removal from water. In this study, MIL-101-NH2 (Al) and MIL-160 (AI) were synthesized and characterized. MIL-101-NH2 (Al) selected as dispersive solid-phase extraction (dSPE) adsorbent, MIL-160 (AI) was used for comparison. The MOF-based dSPE method exhibited excellent efficiency in eluting nucleotides under acidic conditions. Ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) system was used for the determination of AMP, ADP and ATP. A good separation was achieved within 14 minutes, demonstrating high accuracy (82%-121%) and precision (0.4%-14.3%), with a linear range of 1-1000 ng/mL and correlation coefficients (r) > 0.995. The limit of detection (LOD) and limit of quantification (LOQ) were determined to be 0.5 ng/mL and 1 ng/mL, respectively. The MOF-based dSPE and UPLC-MS/MS method developed in this study holds immense potential for further applications in food or biological samples