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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-AM-24
MALDI Glycotyping for O-Antigen Serotyping in Escherichia albertii
(1Hokkaido Univ., 2Miyazaki Pref. Inst. Public Health Environ., 3Akita Pref. Res. Cent. Public Health Environ., 4NIID, 5Jumonji Univ., 6Kagoshima Univ.)
oShogo Urakami1, Yumi Okabe2, Takayuki Konno3, Shinichiro Hirai4, Koichi Murakami5, Tadasuke Ooka6, Hiroshi Hinou1
Escherichia albertii, identified as a new species in 2003, is often misidentified as other species due to its lack of distinctive biochemical characteristics. Some E. albertii strains carry genes for Shiga toxins, posing a risk for severe infections. Despite over 40 reported O-antigen types, only a few have been structurally characterized, and no established diagnostic kits are available. Accurate identification of E. albertii and its serotypes is crucial for tracking infection sources and transmission routes. In this study, we developed a method called MALDI glycotyping to directly detect glycan components of O-antigens, enabling efficient serotyping of E. albertii. This method provides rapid, simple, and parallel analysis of multiple strains, and does not require specific reagents, making it broadly applicable to diverse bacterial species. We applied MALDI glycotyping to analyze 12 E. albertii strains, successfully identifying O-antigen-derived signal patterns in 10 strains. Notably, structural variants were observed in some strains, highlighting the diversity of O-antigen structures. These results demonstrate that MALDI glycotyping is a powerful tool for O-antigen typing and can facilitate the identification of emerging pathogens in the absence of commercial kits.