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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-AM-29
Differential Proteomic Analysis of Microplastic Impact on Mouse Liver Using SWATH-Based Mass Spectrometry
(NTNU)
oPei Chen Lin, Sung-Fang Chen
Plastic pollution presents increasing ecological and health risks, yet the molecular impacts of microplastics on biological systems remain insufficiently explored. Due to the comprehensiveness and flexibility of DIA technology, it is becoming increasingly prominent in proteomic analyses. In this study, a SWATH-based differential proteomics platform was developed to systematically investigate the effects of microplastic exposure on the liver. Through 2D liquid chromatographic separation, 4232 proteins were identified. Of these, 2198 were successfully quantified. A total of 463 proteins showed differential expression in both the low and high-dose treatment groups following microplastic exposure. These proteins are associated with carbon metabolism and the citric acid cycle. A high-coverage proteomic analysis platform was developed in this study and applied to liver tissue from mice exposed to microplastics. This strategy advances our understanding of the molecular disruptions caused by microplastics and offers important insights for monitoring and evaluating potential health risks.