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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-AM-38
Simultaneous Quantification of Antiepileptic Drugs in Serum and Dried Blood Spots using Liquid Chromatography–Tandem Mass Spectrometry
(1KRISS, 2NRC)
Mariam Abady1,2, Ji-Seon Jeong1, oHa-Jeong Kwon1
This study aims to establish an LC-MS/MS method to simultaneously analyze 11 antiepileptic drugs with a particular focus on maintaining accuracy while reducing the number of isotope-labeled internal standards employed for cost-effectiveness. By applying a water/acetonitrile gradient elution containing 0.1% formic acid and 2 mM ammonium formate as the mobile phase, optimal sensitivity for the target drugs could be obtained in positive ESI mode in LC-MS/MS.
In serum samples, extraction with 70% acetonitrile was selected as it provided good recoveries (> 93%) for all targets without matrix effects. Accuracies within 3% were achieved from the combination of six internal standards, while accuracies of 5% and 10% were obtained by reducing the number of internal standards to four and two, respectively, for more economical analysis.
The acetonitrile-based DBS extraction method showed high efficiency for the 11 antiepileptic drugs, providing a simple as well as time- and cost-effective solution. Accuracy and precision within 6% were achieved in both intra- and inter-day assays, with good selectivity, minimal matrix effects, and negligible carryover. A strong correlation between drug concentrations in serum, whole blood, and DBS samples supports the suitability of DBS sampling as a promising and advanced method for antiepileptic drug monitoring.