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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-AM-44
Simultaneous Imaging of Proteins and Metals: Nanoparticle-Based Immunoassay Combined with LA-ICP-MS
(1UTokyo, 2Juntendo Univ., 3Dow Chemical Japan)
oHiroki Nawa1,3, Takehisa Matsukawa2, Ayano Kubota2, Takafumi Hirata1
Metal elements are deeply involved to regulate nearly all biological reactions, and thus interactions between proteins and metals are still key issue to understand biological systems. For the analysis of metal elements, laser ablation-ICP-mass spectrometry (LA-ICP-MS) allows rapid and sensitive analysis, providing spatially resolved elemental data presents in biological tissues. In this study, tagging metals onto proteins through antibody-antigen reaction (i.e. immunoassay) enables two-dimensional imaging of biomolecules, detecting tagged analytes with LA-ICP-MS. To achieve this, we have newly developed highly sensitive technique minimizing non-specific adsorption by means of coating the surface of Au nanoparticles, followed by bioconjugation with antibodies. This method successfully provided the distribution of protein in mouse brain sections, and key elements (Mg, P, Fe, Cu, and Zn) were simultaneously analyzed. With the present technique, elution or secondary diffusion through antibody reaction process was found on several elements. In this presentation, further detailed optimization procedure and biological function of the trace elements, together with effect of buffer solution used in the reaction on elution, will be discussed.