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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-AM-45
Method development for untargeted metabolomics investigating biomarkers for Functional Neurological Disorder in children and adolescents.
(1CFS, UTS, 2C3, UTS, 3Centre for Chemistry, UTS, 4The Children's Hospital at Westmead, Australia)
oRinika Barua1, Shanlin Fu1, Jingyi Yan3, Unnikrishnan Kuzhiumparambil2, Kasia Kozlowska4
Pediatric Functional Neurological Disorder (pFND) is a neuropsychiatric condition caused by complex interactions within the central nervous system and the stress system, manifesting as functional, motor, sensory or cognitive symptoms which compromises the child's ability to function [1,2]. Although there are processes to diagnose a patient [3-5], there is a limitation in understanding the etiology of the disorder. Biomarker discovery using untargeted metabolomics offers the potential to explore the endogenous metabolome of an FND patient to determine the possible causation of the disorder.
Supernatant extracted from blank human plasma was analysed on the Sciex ZenoToF 7600 using a Merck ZIC-HILIC column. Different extraction and reconstitution solvents along with chromatographic and mass spectrometry resolution were investigated to optimise the method. MS-DIAL (version 5.3) was used to determine potential molecular features present and chromatographic separation and resolution provided results to establish a suitable method. Preliminary results determined that a mix of acetonitrile and methanol were a better extraction solvent for plasma samples, along with the use of 10 mM ammonium formate with 90% acetonitrile as a reconstitution solvent, resulting in over 2000 molecular features on MS-DIAL. The method was deemed suitable for conducting untargeted metabolomic analysis of plasma from pFND patients.