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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-AM-46
Development of Isoform-Specific Substrate Peptides for Monitoring PKC Activity
(1Kyoto Univ., 2NCVC, 3NIBIOHN)
oSaki Toi1, Junqi Liang1, Junna Nakazono1, Dai Sakamoto1, Naoyuki Sugiyama1,2, Yasushi Ishihama1,3
Protein phosphorylation is essential for cell signaling, and abnormal phosphorylation can lead to various diseases. Despite over 500 protein kinases in humans, their functions and substrate selectivity remain poorly understood. Phosphoproteome analysis has identified many phosphorylation sites, but the lack of kinase-substrate data limits their utilization. This study aims to develop a method to simultaneously measure kinase activities in human cells using artificial substrate peptides that differentiate similar isoforms. We successfully identified more kinase substrates than previously reported. The motif analysis of the substrate obtained in this study revealed that each PKC isoform has specific substrate motifs. In this presentation, we will also report the evaluation of artificial substrate peptides designed based on the newly discovered isoform-specific substrate motifs.