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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-AM-49
Metabolomic analysis of extracellular vesicles released by cellular senescence
(1Keio Univ., 2NCC)
oRyosuke Hayasaka1, Sho Tabata1,2, Tomoyoshi Soga1, Akiyoshi Hirayama1
Cellular senescence refers to a stable state of cell cycle arrest induced by irreparable DNA damage, such as repeated cell division and oxidative stress. Extracellular vesicles (EVs) are released in large quantities with inflammatory proteins, etc., as senescence-associated secreted phenotype (SASP) in cellular senescence and cause inflammation in surrounding cells. We have developed an analytical system that can sensitively, comprehensively, and quantitatively measure hydrophilic metabolites and lipids contained in EVs from a single sample1,2). However, the differences between the metabolic substances contained in EVs released due to cellular senescence and the profiles within the cells were unknown.
This study elucidates the complete picture of the metabolic substances in the EVs released by senescent cells as SASP. We established a system for inducing replicative senescence in a normal human fibroblast cell line. We then collected EVs under these senescence conditions and performed metabolomic and lipidomic analyses. Metabolites in purine-pyrimidine metabolism in EVs differed depending on cellular senescence. Furthermore, data on hydrophilic metabolites were collected using data-dependent and non-targeted analysis to obtain a wide range of metabolite profiles. Understanding the metabolites in EVs secreted by replicative senescence may lead to a further understanding of cellular senescence.