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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-PM-15
Quantitative Analysis of Endogenous Neuropeptide Y in Developing Brains of Mice and Appetite formation
(1SUNBOR, 2Kanazawa Univ., 3Hamamatsu Univ. of Medicine)
oTohru Yamagaki1, Tomohiro Osugi1, Yohei Shinmyo2,3, Hiroshi Kawasaki2, Honoo Satake1
Neuropeptide Y (NPY) is a central stimulator of food intake and energy expenditure in the hypothalamic nucleus in brain. NPY is consist of 36 amino acids and C-terminal amidation through post-translational modifications. We tried to analyze endogenous NPY directly from developing mice brains quantitatively. There were some issues in the analysis of NPY by using liquid chromatography mass spectrometry (LC-MS) due to the low ionization efficiency of NPY, its adsorption, and the carry-over. We overcame these issues by using blocking peptides of adsorption in high concentration of acetonitrile solutions and using inert columns with superficially porous particles.1,2) When we detected endogenous NPY by LC-MS, gel-filtration chromatography treatment was a critical step to remove other peptides and small metabolites including large amounts of lipids. When we analyzed NPY quantitatively in developing mice brains by microLC triple-quadrupole MS/MS, NPY amounts elevated after birth and the high amounts of NPY were kept in 10 days postnatal brains, which were consistent with the days to form appetite system of NPY in the arcuate nucleus in which NPY nervous system controls appetite. Therefore, our results suggested that birth is a trigger of NPY nervous system formation.3)