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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-PM-19
Development of mass spectrometry-based single-cell multi-omics analysis
(1Kyushu Univ., 2RIKEN CBS, 3YODAKA., 4Niigata Univ., 5RAP)
oKosuke Hata1, Asako Sakaue-Sawano2, Masatomo Takahashi1, Mamoru Hirafuji3, Kohta Nakatani1, Masaki Matsumoto4, Takeshi Bamba1, Atsushi Miyawaki2,5, Yoshihiro Izumi1
The rapid development of next-generation sequencing technology has enabled enomic and transcriptomic analysis of single-cells and has revealed the importance of heterogeneity in biological systems. However, analytical methods to accurately identify and quantify comprehensive proteins and metabolites from single mammalian cells are still under development. To enable proteomic and metabolomic analysis (i.e., multimolecular and phenotypic analysis) from a single animal cell, we have developed i) rapid isolation of target cells under a fluorescence microscope, ii) sample preparation in a small space to reduce sample loss, and iii) ultra-sensitive analysis using nanoflow liquid chromatography tandem mass spectrometry (nano-LC/MS/MS). In this study, we combined “live cell imaging" and “multimolecular and phenotypic analysis" of Fucci3.2 HeLa cells to obtain cell cycle-specific proteomic/metabolomic information at the single-cell level and to quantitatively assess cell heterogeneity.